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mouse alveolar macrophage mh s cells  (ATCC)


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    ATCC mouse alveolar macrophage mh s cells
    PM2.5 triggered ferroptosis and promoted inflammation <t>in</t> <t>MH-S</t> cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in <t>macrophages</t> from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
    Mouse Alveolar Macrophage Mh S Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Atmospheric particulate matter impairs pulmonary barriers by triggering FTH1-mediated ferroptosis"

    Article Title: Atmospheric particulate matter impairs pulmonary barriers by triggering FTH1-mediated ferroptosis

    Journal: iScience

    doi: 10.1016/j.isci.2026.115177

    PM2.5 triggered ferroptosis and promoted inflammation in MH-S cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in macrophages from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
    Figure Legend Snippet: PM2.5 triggered ferroptosis and promoted inflammation in MH-S cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in macrophages from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

    Techniques Used: Immunofluorescence, Expressing, Confocal Laser Scanning Microscopy, Control, Enzyme-linked Immunosorbent Assay, Comparison

    PM2.5 resulted in ferroptosis by upregulating FTH1 in BEAS-2B and MH-S (A) Hub DEGs were identified from the PPI network using Cytoscape, based on the GSE155616 dataset. (B and C) Western blot analysis of FTH1 in PM2.5-induced BEAS-2B and MH-S with or without Fer-1 addition. (C–E) Flow cytometry results of intracellular ROS of BEAS-2B and MH-S cells. (F and G) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of BEAS-2B cells. (H and I) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of MH-S cells. (J and K) Western blot analysis of ZO-1, occludin, and E-cadherin in BEAS-2B. Data are presented as mean ± SEM ( n = 3 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
    Figure Legend Snippet: PM2.5 resulted in ferroptosis by upregulating FTH1 in BEAS-2B and MH-S (A) Hub DEGs were identified from the PPI network using Cytoscape, based on the GSE155616 dataset. (B and C) Western blot analysis of FTH1 in PM2.5-induced BEAS-2B and MH-S with or without Fer-1 addition. (C–E) Flow cytometry results of intracellular ROS of BEAS-2B and MH-S cells. (F and G) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of BEAS-2B cells. (H and I) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of MH-S cells. (J and K) Western blot analysis of ZO-1, occludin, and E-cadherin in BEAS-2B. Data are presented as mean ± SEM ( n = 3 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

    Techniques Used: Western Blot, Flow Cytometry, Comparison



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    PM2.5 triggered ferroptosis and promoted inflammation <t>in</t> <t>MH-S</t> cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in <t>macrophages</t> from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
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    PM2.5 triggered ferroptosis and promoted inflammation <t>in</t> <t>MH-S</t> cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in <t>macrophages</t> from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
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    PM2.5 triggered ferroptosis and promoted inflammation <t>in</t> <t>MH-S</t> cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in <t>macrophages</t> from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
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    PM2.5 triggered ferroptosis and promoted inflammation <t>in</t> <t>MH-S</t> cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in <t>macrophages</t> from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.
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    Image Search Results


    PM2.5 triggered ferroptosis and promoted inflammation in MH-S cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in macrophages from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

    Journal: iScience

    Article Title: Atmospheric particulate matter impairs pulmonary barriers by triggering FTH1-mediated ferroptosis

    doi: 10.1016/j.isci.2026.115177

    Figure Lengend Snippet: PM2.5 triggered ferroptosis and promoted inflammation in MH-S cells (A) Immunofluorescence analysis and quantification of CD68 expression (white arrows) in the lung tissues of mice subjected to various exposure patterns. Scale bars: 50 μm. Red arrows highlight the particle aggregates. (B) Confocal laser scanning microscopy images illustrating the phagocytic capacity of MH-S following PM2.5 exposure at various time intervals. Yellow arrowheads denote particle aggregates, and white arrowheads indicate filopodia protrusions. Scale bars: 50 μm. (C) The average number of pHrodo green Zymosan bioparticles ingested per cell. (D) The percentage of cells containing pHrodo green Zymosan bioparticles. (E–G) Volcano plots, KEGG enrichment, and GO analysis of DEGs in macrophages from PM2.5 exposure and Control groups in a dataset ( GSE294723 ). (H) Level of MDA in MH-S treated with PM2.5 (20 and 50 μg/cm 2 ) with or without the addition of Fer-1 (5 μM) for 6 h. (I) qPCR analysis of GPX4, SLC7A11, Nrf2, and SOD2. (J and K) IL-6 and TNF-α levels in MH-S detected by ELISA. (L–N) Immunofluorescence images and quantitative assessments of the phagocytic capacity of MH-S treated with PM2.5 (50 μg/cm 2 ) with or without Fer-1 (5 μM) for 6 h. Scale bars: 50 μm. Data are presented as mean ± SEM ( n = 4 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

    Article Snippet: Mouse alveolar macrophage MH-S cells (ATCC, CRL-2019) were maintained in RPMI-1640 medium supplemented with 10% FBS (ExCell Bio, Suzhou, China).

    Techniques: Immunofluorescence, Expressing, Confocal Laser Scanning Microscopy, Control, Enzyme-linked Immunosorbent Assay, Comparison

    PM2.5 resulted in ferroptosis by upregulating FTH1 in BEAS-2B and MH-S (A) Hub DEGs were identified from the PPI network using Cytoscape, based on the GSE155616 dataset. (B and C) Western blot analysis of FTH1 in PM2.5-induced BEAS-2B and MH-S with or without Fer-1 addition. (C–E) Flow cytometry results of intracellular ROS of BEAS-2B and MH-S cells. (F and G) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of BEAS-2B cells. (H and I) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of MH-S cells. (J and K) Western blot analysis of ZO-1, occludin, and E-cadherin in BEAS-2B. Data are presented as mean ± SEM ( n = 3 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

    Journal: iScience

    Article Title: Atmospheric particulate matter impairs pulmonary barriers by triggering FTH1-mediated ferroptosis

    doi: 10.1016/j.isci.2026.115177

    Figure Lengend Snippet: PM2.5 resulted in ferroptosis by upregulating FTH1 in BEAS-2B and MH-S (A) Hub DEGs were identified from the PPI network using Cytoscape, based on the GSE155616 dataset. (B and C) Western blot analysis of FTH1 in PM2.5-induced BEAS-2B and MH-S with or without Fer-1 addition. (C–E) Flow cytometry results of intracellular ROS of BEAS-2B and MH-S cells. (F and G) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of BEAS-2B cells. (H and I) Western blot analysis and quantitative results of GPX4, SLC7A11, and HO-1 of MH-S cells. (J and K) Western blot analysis of ZO-1, occludin, and E-cadherin in BEAS-2B. Data are presented as mean ± SEM ( n = 3 independent experiments). ∗ p < 0.05 and ∗∗ p < 0.01 as determined by two-way ANOVA followed by Dunnett’s multiple comparison test.

    Article Snippet: Mouse alveolar macrophage MH-S cells (ATCC, CRL-2019) were maintained in RPMI-1640 medium supplemented with 10% FBS (ExCell Bio, Suzhou, China).

    Techniques: Western Blot, Flow Cytometry, Comparison